Diagnostic Accuracy of Different Histological Stains for Helicobacter Pylori Detection, taking Immunohistochemistry as Gold Standard

Aim: Helicobacter pylori infection has been ascertained to play pivotal role in the pathogenesis of chronic gastritis and gastric neoplasia.1The present study was performed evaluate diagnostic accuracy H&E stain Giemsa for histological diagnosis helicobacter by taking immunohistochemical staining as a gold standard. Methods: A total 155 cases were included our study. The received biopsies fixed 10% buffered formalin, grossed stained with giemsa stain. board histopathologists analyzed morphological details ascertain diagnosis. using techniques against H. antigens, procedure according guidelines provided manufacturer considering appropriate positive negative controls staining. IHC evaluated autonomously recorded on proforma cases. Results: In study, mean age calculated 38.4±11.57 years, 74(47.74%) male 81(52.26%) females, frequency H.Pylori standard 109(70.32%), hematoxylin-eosin detection measure 63.30%, 65.22%, 81.18%, 42.86% 63.87% sensitivity, specificity, predictive value, value rate respectively, while these findings 74.31%, 80.43%, 90%, 56.92% 76.12% Conclusion: We concluded that stains HP is promising cost-effective method population. MeSH words: pylori, Immunohistochemistry, Hematoxylin, Pathology, Diagnosis